Immune deficient mice growing xenografts of HT-29 or A-431 cell lines were treated with cisplatin, carboplatin or doxorubicin in combination with one hour of wholebody pulsed magnetic field (PMF) exposure (calculated peak field 5.2 m Tesla, with an averagefield strength of 0.525 m Tesla-rms; pulses rose for 120 mu-sec and then abruptly fell to neutral, and were repeated at a rate of 250 pulses per second). At 24 days, the mice in each experiment were found to have significantly (p lt 0.05, ANOVA) different tumor sizes among groups. The smallest mean tumor volume was consistently found in the drug+PWF group. With A431 tumors, the cisplatin + PMF group (T) was significantly smaller, 52% (1-(100T/C)), than the cisplatin alone group (C). In HT-29 tumors, those treated with carboplatin + PMF had the smallest tumor volume at just 34% of the carboplatin-alone group. In HT-29
tumors, the doxorubicin + PMF group was 35% of the doxorubicin alone group.

DESCRIPTOR(S)- *ANTINEOPLASTIC-DRUG; *CARBOPLATIN; *CISPLATIN;
*DOXORUBICIN; *MOUSE; * THERAPEUTIC METHOD BIOSIS Concept Code(s)-
10606; 11104; 11107; 22005; 24004; 24008 BIOSYSTEMATIC CODES-
86215; 86375 Secondary Concept Code(s)- 10060; 12512.

Effect of pulsed magnetic fields on human umbilical endothelial vein cells 

BIO 97-05 97-042417 1

Goodman, E. M.; Greenebaum, B.; Frederiksen, J.
JOURNAL NAME- Bioelectrochemistry and Bioenergetics VOL. 32 NO. 2
1993 PP. 125-132 ISSN- 0302-4598 AUTHOR AFFILIATION- Biomedical
Res. Inst., Univ. Wisconsin-Parkside, Kenosha, WI 53141, USA LITERARY
INDICATOR(S)- RESEARCH ARTICLE PRINT PRODUCT NUMBER- Biological
Abstracts Vol. 097 Iss. 003 Ref. 026717 LANGUAGE- English

The long-term goal of this program was to examine the efficacy of weakelectromagnetic fields as an adjunct therapy to facilitate wound repair.The experiments reported here were directed to the question of whetherpulsed magnetic fields (PMFs) could facilitate endothelial cell migrationfollowing induction of a wound. These studies were based on a report thatweak PMFs facilitated cell migration in a wounded confluent monolayerculture of human umbilical vein cells. Experiments reported in this paperwere an attempt to reproduce and extend these observations. Our data
support the original observations. Further, we examined severalhypotheses in an attempt to clarify the mechanism of interaction betweenthe applied electromagnetic fields and cells. The data do not support thehypothesis that PMFs are acting in a manner analogous to endothelial cellgrowth factor, a normal component of the growth medium, or that PMFsapplied at the time of cell transfer enhance DNA replication. We havefound that PMFs are more effective in accelerating migration if theyinduce an electric field that is perpendicular rather than parallel tothe sides of the wound.

DESCRIPTOR(S)- *CELL MIGRATION; * THERAPY PLANNING; *WOUND REPAIR
BIOSIS Concept Code(s)- 02508; 11107; 12512; 14508 BIOSYSTEMATIC

CODES- 86215 Secondary Concept Code(s)- 10610; 12100.

EFFECTS OF PULSED EXTREMELY-LOW-FREQUENCY MAGNETIC FIELDS ON SKIN 
WOUNDS IN THE RAT -
BO1 BA 85-00 BA 85-090642
OTTANI, V.; DE PASQUALE, V.; GOVONI, P.; FRANCHI, M.; ZANIOL, P.;
RUGGERI, A.

ABBREVIATED JOURNAL TITLE- BIOELECTROMAGNETICS VOL. 9 NO. 1 1988
53-62 page(s) CODEN- BLCTD AUTHOR AFFILIATION- IST. ANAT. UMANA
NORMALE, VIA IRNERIO 48, 40126 BOLOGNA, ITALY. SUBFILE- BA (Biological
Abstracts) LANGUAGE- English
NO-ABSTRACT
DESCRIPTOR(S)- *BACK; * CELLULAR ; *COLLAGEN; *MATURATION; *NETWORK;
*NEW; *ORGANIZATION; *VASCULAR BIOSIS Concept Code(s)- 06506; 10610;
11107; 14504; 18506; 25508 BIOSYSTEMATIC CODES- 86375 Secondary
Concept Code(s)- 06504; 11104; 11310.

PULSED ELECTRO MAGNETIC FIELD THERAPY OF PERSISTENT ROTATOR CUFF 
TENDINITIS A DOUBLE-BLIND CONTROLLED ASSESSMENT- BO1 BA 78-00 BA
78-032801 8

BINDER, A.; PARR, G.; HAZLEMAN, B.; FITTON-JACKSON, S.
ABBREVIATED JOURNAL TITLE- LANCET VOL. 1 NO. 8379 1984 695-698
page(s) CODEN- LANCA AUTHOR AFFILIATION- DEP. RHEUMATOL.,
ADDENBROOKE'S HOSP., CAMBRIDGE, CB2 2QQ. SUBFILE- BA (Biological
Abstracts) LANGUAGE- English

NO-ABSTRACT

DESCRIPTOR(S)- *CHRONIC; *HUMAN; *LESION; *STEROID; *TENDON;
* THERAPY BIOSIS Concept Code(s)- 17506; 18006 BIOSYSTEMATIC CODES-
86215 Secondary Concept Code(s)- 10057; 10067; 10610; 12504; 12508;
17501; 18001.

BIOCHEMICAL ASPECTS OF THE BIOLOGICAL EFFECT OF LOW FREQUENCY PULSED
ELECTRO MAGNETIC FIELD

- BO2 BA 55-00 BA 55-020742
KOLODUB, F. A.; EVTUSHENKO, G. I.
ABBREVIATED JOURNAL TITLE- GIG TR PROF ZABOL VOL. 16 NO. 6 1972
13-17 page(s) CODEN- GTPZA SUBFILE- BA (Biological Abstracts)
NO-ABSTRACT

DESCRIPTOR(S)- *RAT BIOSIS Concept Code(s)- 10060; 10502; 10610;
12002; 13002; 13003; 13004; 13012 BIOSYSTEMATIC CODES- 86375

Secondary Concept Code(s)- 10062; 10064; 10068; 10510; 13014; 14004;
14504; 17504; 20504.

Excitation of frog sciatic nerve using pulsed magnetic fields effect of waveform variations 

- INS 97-39 5709110 A9721-8730-021
(PHA) 0

Alkhateeb, A.; Gaumond, R. P.

ABBREVIATED JOURNAL TITLE- 1995 IEEE Engineering in Medicine and Biology
17th Annual Conference and 21 Canadian Medical and Biological Engineering
Conference (Cat. No.95CH35746) PART NUMBER- vol.2 1997 PP. 1119-20
vol.2 2 vol. ixviii+1738 page(s) 3 reference(s) DOCUMENT TYPE-
Conference paper ISBN- 0 7803 2475 7 CORPORATE AUTHOR- Dept. of
Bioeng., Penn State Univ., University Park, PA, USA COPYRIGHT OF
BIBLIOGRAPHIC- Copyright 1997, IEE COPYRIGHT CLEARANCE CENTER CODE- 0
7803 2475 7/97/$10.00 PUBLISHER- IEEE PUBLICATION PLACE- New York,
NY, USA PUBLICATION COUNTRY- USA CONFERENCE DATE- 20-23 Sept. 1995
CONFERENCE TITLE- Proceedings of 17th International Conference of the

Engineering in Medicine and Biology Society CONFERENCE LOCATION-
Montreal, Que., Canada LANGUAGE- English (DEF)
We examined the relative effectiveness of overdamped and of (truncated)underdamped magnetic field pulses in exciting frog sciatic nerve.Waveforms were compared by finding the stimulus magnitude (Vh)corresponding to half-maximal tension developed in gastroenemius muscle.Vh varied inversely with T/sub 0/ the time to peak of the magnetic field.

DESCRIPTOR(S)- biological effects of fields; biomagnetism; muscle;
neurophysiology; zoology IDENTIFIER(S)- frog sciatic nerve excitation;
gastroenemius muscle; half-maximal tension; magnetic induction;
overdamped magnetic field pulses; pulsed magnetic fields; stimulus
magnitude; time to peak; truncated underdamped magnetic field pulses;
waveform variations TREATMENT CODE- TC-X SECTIONAL CLASSIFICATION
CODE- A8730E; A8740; A8750; A8760D.

Stimulation of nerves and muscles using large pulsed magnetic fields 

- INS 95-49 5139121 A9602-8760D-003 (PHA)
Barker, A. T.

ABBREVIATED JOURNAL TITLE- IEE Colloquium on 'Magnets in Medicine -
Hazards and Health Care' (Digest No.1995/164) 1995 PP. 1/1-3 24
page(s) 3 reference(s) DOCUMENT TYPE- Conference paper CORPORATE
AUTHOR- Dept. of Med. Phys. & Clinical Eng., R. Hallamshire Hospital,
Sheffield, UK SPONSORING AGENCY- IEE COPYRIGHT OF BIBLIOGRAPHIC-
Copyright 1995, IEE PUBLISHER- IEE PUBLICATION PLACE- London, UK
PUBLICATION COUNTRY- UK CONFERENCE DATE- 9 Oct. 1995 CONFERENCE
TITLE- IEE Colloquium on 'Magnets in Medicine - Hazards and Health Care'
(Digest No.1995/164) CONFERENCE LOCATION- London, UK LANGUAGE-
English (DEF)

It has been known since the work of Galvani and Volta in the 1790s thatnerves and muscles can be electrically stimulated. The technique ofelectrical stimulation, using either needle or surface electrodes toinject current into the tissue, is today widely used in medicine both fordiagnostic purposes and for other applications such as cardiac pacing,defibrillation of the heart and to aid gait. It does, however, have somedisadvantages and limitations, in particular the need to use needleelectrodes to stimulate deep nerves, its relative inability to stimulatestructures surrounded by bone such as the human brain and the discomfortit causes. Over 2000 magnetic stimulators are now in use world-wide forboth clinical and research applications. The technique has becomeestablished as a diagnostic tool in neurology and the recent emergence ofa number of potential therapeutic applications suggests that the use of
magnetic stimulation in medicine is likely to grow.

DESCRIPTOR(S)- biological effects of fields; muscle; neurophysiology;
patient diagnosis; radiation therapy ; reviews IDENTIFIER(S)-
clinical applications; diagnostic tool; large pulsed magnetic fields;
magnetic stimulation; magnetic stimulators; muscle stimulation; nerve
stimulation; neurology; research applications TREATMENT CODE- TC-G
SECTIONAL CLASSIFICATION CODE- A8760D; A8730; A0130R; A8740; A8770E;
A8770H.

- INS 93-43 4515653 A9324-8750G-007 (PHA)
Svedenstal, B.-M.; Holmberg, B.
JOURNAL NAME- International Journal of Radiation Biology ABBREVIATED
JOURNAL TITLE- Int. J. Radiat. Biol. (UK) VOL. 64 NO. 1 July 1993
PP. 119-25 21 reference(s) DOCUMENT TYPE- Journal paper ISSN-
0020-7616 CODEN- IJRBA3 CORPORATE AUTHOR- Dept. of Radioecology,
Swedish Univ. of Agric. Sci., Uppsala, Sweden COPYRIGHT CLEARANCE
CENTER CODE- 0020-7616/93/$10.00 PUBLICATION COUNTRY- UK LANGUAGE-
English (DEF)

CBA mice were exposed to a total of 5.24 Gy X-rays (260 kV, 11 mA, 0.45Gy/min) divided into four exposures, and to saw-tooth 15 mu T (peak topeak) pulsed vertical 20 kHz magnetic fields for their life-time. Inparallel, series with magnetic fields only or non-exposed animals wererun. The animals were observed for their life-time. The frequency oflymphomas was 65.7% in the X-ray group and 71.4% in the groups exposed toboth X-ray and magnetic field. Of the non-exposed control animals, and ofthe exposed to magnetic fields only 6.4 and 5.7% had lymphomas,
respectively. There were no statistically significant differences betweenthe magnetic field series and the corresponding controls for lymphomas.Blood cell counts and haemoglobin data did not show differences between
the X-irradiated, and X-ray and magnetic field-exposed groups.Mononuclear cells and total leukocytes were elevated for pulsed magneticfields-exposed animals compared with the non-exposed controls. Thisdifference was due to two animals with extreme values. In the pulsedmagnetic fields treated group there was a statistically significantincrease of the carcass weight compared with the non-treated controlgroup.

DESCRIPTOR(S)- biological effects of fields; biological effects of
X-rays; biomagnetism; blood; cellular effects of radiation;
magnetic field effects IDENTIFIER(S)- blood cell counts; carcass
weight; haemoglobin; lymphoma development; mononuclear cells; pulsed
magnetic fields; radiation biology ; total leukocytes; CBA mice;
X-ray exposure; 11 mA; 15 muT; 20 kHz; 260 kV NUMERICAL DATA
INDEXING- voltage 2.6E+05 V; current 1.1E-02 A; magnetic flux density
1.5E-05 T; frequency 2.0E+04 Hz TREATMENT CODE- TC-X SECTIONAL
CLASSIFICATION CODE- A8750G; A8725; A8750; A8740.

INS 91-19 3961194 A91118579 (PHA) 0 Ueno, S.; Hiwaki, O.
JOURNAL NAME- Journal of Applied Physics ABBREVIATED JOURNAL TITLE- J.
Appl. Phys. (USA) pt.2B VOL. 69 NO. 8 15 April 1991 PP. 6019-21
17 reference(s) DOCUMENT TYPE- Conference paper ISSN- 0021-8979
CODEN- JAPIAU CORPORATE AUTHOR- Dept. of Electron., Kyushu Univ.,
Fukuoka, Japan SPONSORING AGENCY- AIP; IEEE; et al COPYRIGHT
CLEARANCE CENTER CODE- 0021-8979/91/086019-03$03.00 PUBLICATION
COUNTRY- USA CONFERENCE DATE- 29 Oct.-1 Nov. 1990 CONFERENCE TITLE-
Thirty-Fifth IEEE Conference on Magnetism and Magnetic Materials
CONFERENCE LOCATION- San Diego, CA, USA LANGUAGE- English (DEF)

A noninvasive method of magnetic stimulation of the spinal roots wasdesigned. The basic idea is to concentrate induced eddy currents in atarget by a pair of opposing pulsed magnetic fields. A figure-eight coil
was positioned outside the median of the back so that time varyingmagnetic fields pass through the body in opposite directions around thetarget. Magnetic stimulation of the spinal roots of human and a rabbitwas carried out. It was found that each spine level can be stimulatedselectively, producing electromyographic waves related to both the
H-reflex and M-wave. The results indicate that the M-wave can be producedby currents flowing either in the rostral or caudal direction, whereasthe H-reflex is only generated by currents flowing in the caudaldirection. The H-reflex elicited by magnetic stimulation of nerves in thevicinity of the spine becomes a new tool in diagnosis of neuromuscularsystem diseases.
DESCRIPTOR(S)- bioelectric phenomena; biological effects of fields;
biomagnetism; magnetic field effects; neurophysiology IDENTIFIER(S)-
caudal direction; concentrate induced eddy currents; electromyographic
waves; figure-eight coil; human; magnetic stimulation; neuromuscular
system diseases; noninvasive method; opposing pulsed magnetic fields;
rabbit; rostral; spinal reflex; spinal roots; spine level; time
varying magnetic fields; H-reflex; M-wave TREATMENT CODE- TC-X
SECTIONAL CLASSIFICATION CODE- A8740; A8728; A8750; A8730C.

- INS 91-16 3929295 A91099541 (PHA)
Smith, O. M.; Goodman, E. M.; Greenebaum, B.; Tipnis, P.
JOURNAL NAME- Bioelectromagnetics ABBREVIATED JOURNAL TITLE-
Bioelectromagnetics (USA) VOL. 12 NO. 3 1991 PP. 197-202 16
reference(s) DOCUMENT TYPE- Journal paper ISSN- 0197-8462 CODEN-
BLCTDO CORPORATE AUTHOR- Biomed. Res. Inst., Wisconsin Univ., Parkside,
Kenosha, WI, USA PUBLICATION COUNTRY- USA LANGUAGE- English (DEF)

Pulsed magnetic fields have been used to enhance healing of bonefractures and purportedly of lesions in soft tissue. However, theirmechanism of action is poorly understood. The authors report changes inthe plasma membrane of a nonadherent mammalian cell line, U937, which wasexposed to a 25-pps magnetic field for 48 hours. Aqueous polymertwo-phase partition studies showed that magnetic-field-exposed cellsexhibited an increased negative surface charge but membranehydrophobicity was not significantly altered. The observed increase inmembrane electronegativity of exposed cells did not reflect a significantchange in growth rate.

DESCRIPTOR(S)- bioelectric phenomena; biological effects of fields;
biomagnetism; biomembranes; cellular effects of radiation; magnetic
field effects IDENTIFIER(S)- aqueous polymer 2-phase partition studies;
bone fracture healing; membrane electronegativity; membrane
hydrophobicity; negative surface charge; nonadherent mammalian cell
line; plasma membrane; pulsed magnetic field; soft tissue lesions;
U937 cells; 48 hr NUMERICAL DATA INDEXING- time 1.7E+05 s TREATMENT
CODE- TC-X SECTIONAL CLASSIFICATION CODE- A8728; A8725F; A8740;
A8750; A8720E.